MicroRNA-126은 HIF-1α의 발현을 통해 구강 편평상피세포암의 증식능과 이주능을 촉진한다
MicroRNA-126 Promotes the Proliferation and Migration of Oral Squamous Cell Carcinoma Cells Via HIF-1α Expression
박수연, 오지현, 이상신, 최종호,
소속 상세정보
박수연 ( Park Su-Yeon ) - Gangneung-Wonju National University College of Dentistry Department of Pathology
오지현 ( Oh Ji-Hyeon ) - Gangneung-Wonju National University College of Dentistry Department of Oral and Maxillofacial Surgery
이상신 ( Lee Sang-Shin ) - Gangneung-Wonju National University College of Dentistry Department of Pathology
최종호 ( Choi Jong-Ho ) - Gangneung-Wonju National University College of Dentistry Department of Pathology
Abstract
Oral squamous cell carcinoma (OSCC) is the most common type of head and neck cancer and is associated with high recurrence, poor treatment, and low survival rates. Hypoxia-inducible factor-1α (HIF-1α) is a transcription factor that regulates the response to hypoxia, a major factor in the tumor microenvironment that affects tumor development and progression in various cancer types. However, microRNA (miRNA) sequence analysis revealed that only a few miRNAs targeting HIF-1α had been discovered. In the present study, we investigated HIF-1α expression in OSCC and the effect of HIF-1α-targeting miRNAs on the progression and metastatic potential of OSCC. We analyzed public databases to explore which miRNAs target HIF-1α expression. In addition, the expression of proteins involved in the cell cycle, proliferation, and apoptosis in HSC-2 cells was analyzed after miRNA-126 mimic treatment. Furthermore, to investigate the effect of miRNA-126 on the proliferation and invasion ability of OSCC cells, 5-ethynyl-2′-deoxyuridine and Transwell assays were performed. The activities of MMP-2 and MMP-9 were evaluated via gelatin zymography. Our results showed that miRNA-126, which targets HIF-1α, enhances OSCC cell proliferation by regulating the cell cycle and reinforces the cell mobility of OSCC via HIF-1α expression. These findings suggest that miRNA-126 may be a novel marker for OSCC treatment and the development of new tools for patients with OSCC.
키워드
Oral squamous cell carcinoma; MicroRNAs; HIF-1α; Metastasis; Proliferation
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